Build the science that shapes the future of human health.
Application closing date: 08.03.2026

Join a place where ambitious science thrives
Human Technopole (Milan) is a rapidly expanding life science institute where international researchers and cutting-edge technologies converge to accelerate biomedical discovery. Our mission is to transform bold scientific ideas into advances that improve human health.

Within this mission, The Structural Proteomics Unit studies protein structure and interactions using advanced mass spectrometry–based proteomics, with a strong focus on crosslinking MS and high-throughput workflows, including single-cell proteomics. It operates state-of-the-art instrumentation and advanced data analysis pipelines within the multidisciplinary ecosystem of Human Technopole. The Proteomics Specialist will report to the Unit Manager and collaborate closely with National Facilities and research groups.

Your mission

• Develop and optimize advanced proteomics workflows
  Establish robust protocols for single-cell, spatial/visual, plasma and clinical proteomics, including bottom-up sample preparation pipelines and peptide enrichment strategies.
• Operate and maintain mass spectrometry platforms
  Perform instrument calibration, cleaning, basic maintenance, and support automation of peptide extraction, digestion, and cleanup for diverse sample types.
• Manage large-scale proteomics studies
  Oversee processing and data acquisition for high-throughput projects involving hundreds to thousands of samples, ensuring quality and reproducibility.
• Analyze data and support facility users
  Deliver and explain results to users, contribute to experimental design and project planning, provide technical support during projects, and train internal and external researchers.
• Contribute to the growth of the Unit
  Support resource planning, introduce new protocols and applications, collaborate with vendors and service engineers, mentor junior staff, and participate in facility reviews and research initiatives.

Grow your skills
You will enhance your scientific and professional skills by working with:

• cutting-edge proteomics technologies, including state-of-the-art mass spectrometry platforms, automation, and advanced workflows for single-cell, spatial, and clinical proteomics.
• complex experimental design and large-scale data generation, ensuring high-quality, reproducible results in a high-throughput facility environment.
• multidisciplinary collaboration and facility operations, developing expertise in project coordination, user support, technology development, and mentoring within a leading research infrastructure.

Human Technopole supports career development through training, mentoring and dedicated learning opportunities.

What you'll bring

Essential

• PhD in a relevant subject (e.g. biology, biotechnology, biochemistry, chemistry) to be obtained within the deadline of the call.

• At least 5 years of prior experience with bottom-up proteomics by mass spectrometry (this can include PhD years) including sample preparation.
• Fluency in English – HT is an international research institute.
• Familiarity with proteomics search engines (MaxQuant, FragPipe, Dia-NN, Spectronaut or similar).
• At least one of the following: experience with plasma proteomics, spatial/tissue proteomics, clinical proteomics, single cell proteomics, establishing proteomics sample prep automation.
• Experiencing with implementing mass spec/proteomics methods at the spectrometer.
• Familiarity with operating and troubleshooting LC-MS platforms.

Preferred

Any of the following:

• Experience with DIA for global untargeted proteomics

• Familiarity with Astral, Hybrid or Tribrid Mass Spectrometers.
• Experience in working with large cohorts and/or patient sample sets, including benchmarking and QC.
• Experience in standardising workflows and/or developing targeted assays.
• Experience in glycoproteomics, including data analysis
• Experience in metabolomics, including data analysis
• Experience in lipidomics, including data analysis
• Experience with machine learning in proteomics data analysis.

Organizational and social skills:

• Communication, team building and problem-solving skills;
• Ability to interact effectively with other teams and work synergistically to effectively drive projects.
• Highly organised and meticulous work attitude.
• Motivation to mentor and train.

At HT, your discoveries contribute to a global effort to improve human health.

Why Human Technopole
HT offers an international and dynamic workplace, competitive welfare provisions, flexible working policies and relocation support. Researchers relocating to Italy may benefit from attractive tax benefits. We promote work–life balance and provide parental support initiatives.

How to apply
Submit:
• CV
• Motivation letter (English) including names of MS instruments you are familiar with
• Contact details of two referees

This is a 3-year contract offered under CCNL Chimico Farmaceutico, Level B2

Salary: up to € 54.000,00 depending on the candidate’ seniority.
The position is based in Milan, Italy, within our vibrant international campus.
We strongly encourage applications from candidates belonging to protected categories (L. 68/99).